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Biotechnology—Bio-Rad Classroom Kits
Out of the Blue CRISPR Kit and Genotype Extension
Use CRISPR-Cas9 gene editing to edit the lacZ gene in E. coli. Visualize results with blue-white screening and confirm them by PCR.
Deepen your understanding of CRISPR, its promise and risks, and the ethics behind its many applications.
Perform true CRISPR gene editing in your laboratory class!
CRISPR gene editing is revolutionizing scientific research. Now your students can do real CRISPR-Cas9 gene editing in the classroom
using Bio-Rad’s educational CRISPR gene editing kits.
• Out of the Blue CRISPR Kit – Apply CRISPR-Cas9 technology to disrupt the lacZ gene in E. coli and produce a phenotype that can be
readily visualized through blue-white screening.
• Out of the Blue CRISPR Genotyping Extension – (Optional) Confirm the edit at the molecular level using PCR and argarose gel
electrophoresis.
The Out of the Blue CRISPR and Genotyping Kits earned the 2020 Science Educators’ Best of Show award for “Best New Technology
Innovation for Science” from the National Science Teaching Association (NSTA).
• Hands-on, true CRISPR gene editing – Students get direct experience with this powerful technology
• Visible phenotype – Results can be observed with the naked eye in as little as 22 hours.
• Safe – Familiar and common reagents and non-pathogenic, non-biofilm-forming bacteria generate a phenotype that is common to
molecular biology.
• Accessible – Does not require specialized equipment or techniques.
• Comprehensive, supportive curriculum – Includes background about the components of a CRISPR-Cas9 system, the science and
math behind its accuracy and flexibility, and the guide bioinformatic analysis of CRISPR targets students design themselves.
FB2579, etc.
Out of the Blue CRISPR Kit
and Genotyping Extension
Use paper model
to understand
gene editing
PAM
Edit lacZ gene
Genotype results
(optional)
Conduct
bioinformatics
capstone activity
Out of the Blue Genotyping Extension
Kit contains sufficient materials for 8 student workstations (2–4 students per
workstation).
Description Qty
Out of the Blue Primer Mix (50x) 1
Out of the Blue PCR Positive Control DNA 1
PCR Master Mix (Taq DNA Polymerase, dNTPs, Buffers), 1.2 mL 1
PCR Molecular Weight Ruler 1
Orange G Loading Dye 1
InstaGene Matrix, 20 mL 1
PCR Tubes, 0.2 mL 100
Microentrifuge Tubes, 1.5 mL with Caps 60
Conical Tubes, 1.5 mL, Sterile 50
Instructor Answer Guide 1
Out of the Blue CRISPR Kit
Kit contains sufficient materials for 8 student workstations (2–4 students per
workstation).
Description Qty
E. coli HB 101-pBRKan, Lyophilized 1
Donor Template DNA (pLZDonor) 1
Donor Template DNA and Guide RNA (pLZDonorGuide) 1
KIX Mix 1
Spectinomycin Powder 1
Arabinose, Lyophilized 1
Sterile Transformation Solution 1
LB Nutrient Agar Powder 1
LB Broth Capsule, Makes 50 mL LB Broth 1
Inoculation Loops, Sterile 80
Petri Dishes, Sterile 60
2.0 mL Microcentrifuge Tubes, Sterile 90
Easy Start Guide 1
Instructor Answer Guide 1
BIOTECHNOLOGY—BIO-RAD continued on next page.
Out of the Blue CRISPR Kit and
Genotyping Extension
Bio-Rad Explorer kits address the critical need for inquiry-based activities—an
important component of scientific literacy and a launch point of experience and
practical training for students interested in careers in life science. The kits range
from introductory to advanced topics, including courses guiding students through
entire molecular biology workflows.
Catalog No. Description Material No. Price/Each
FB2579 Out of the Blue CRISPR Kit and
Genotyping Extension
17006081EDU $427.73
FB2442 Out of the Blue CRISPR Kit 17006284EDU 299.09
FB2504 Out of the Blue Genotyping Extension 12012607EDU 213.33
FB2332 Out of the Blue CRISPR Kit Refill Pack 12012620EDU 213.33
FB2333 Out of the Blue Genotyping
Extension Refill Pack
12012708EDU 191.89
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