flinnsci.com 483
Drosophila Culture Vials
and Foam Plugs
AB1423 and AB1392
Animals—Care Supplies, Invertebrates,
continued
Drosophila See page 471.
Vials, Drosophila Culture
Clear, polystyrene vials. Vials measure 4" H x 11⁄4" W—the standard size for Drosophila
culture. Foam plugs (available separately) fit snugly in place, allowing cultures to
breathe. Vials can be sterilized with a dilute bleach solution if desired. Supplied in
packages of 10.
Catalog No. Description Price/Pkg. of 10
AB1423 Vials, Drosophila Culture $8.59
Plugs, Foam, White
Plastic foam plugs are autoclavable and reusable. Plugs throw no fibers or lint and
allow cultures to breathe. Will fit containers with mouth diameters of 1" to 13 ⁄8" (27–34
mm). Supplied in packages of 10.
Catalog No. Description Container Diam. Price/Pkg. of 10
AB1392 Plugs, Foam, White 27–34 mm $5.45
Anesthetizer,
Lull-A-Fly™ Kit
FB1438
Anesthetizer, Lull-A-Fly™ Kit
By: The Flinn Staff
Lull-A-Fly™ will anesthetize Drosophila for up to 45 minutes without killing or sterilizing
them. Besides working like a charm, Lull-A-Fly is a much safer alternative than
ether. The small-tipped applicator allows anesthetization right in culture bottles, and
the amount required is minimal and, therefore, less expensive.
Each kit contains 15 mL of Lull-A-Fly (150 doses), 15 anesthetic wands, a morgue
container and complete directions for use.
See free video at flinnsci.com.
Catalog No. Description Price/Each
FB1438 Lull-A-Fly™ Kit $12.85
Animals—Care Supplies, Protists
Protozoan Culture Media
Catalog No. Description Package Size Price/Each
FB1820 Bristol’s Algae Media Concentrate 100 mL $8.74
Quieting/Slowing Solution for Protozoa
Nontoxic methyl cellulose (3.0%) solution for slowing the movements of protozoa. The
high viscosity of a drop of this solution added to a wet-mount preparation will slow
(or cease) movement while students make careful observations.
Catalog No. Description Pkg. Size Price/Each
M0171 Quieting/Slowing Solution for Protozoa 100 mL $9.03
Bristol’s Algae
Media Concentrate
FB1820
Quieting/Slowing
Solution for Protozoa
M0171
Prepare Infusion Media for Protozoans
Culture media made by soaking or heating fresh or dried organic (usually plant) material in
water are known as “infusions.” Organic materials used include hay, wheat seed, rice and
oat flakes—either individually or in some combination.
The source of the water used is critical. Spring water, well water and filtered water from
unpolluted streams or ponds are the best choices. The ratio of organic material should be
approximately 7 grams (0.25 ounces) per liter.
To pre-treat the organic material, place it in boiling water for one to two minutes, remove
it and maintain it under sterile conditions until it is needed. When you are ready to set up
the cultures, pour one inch of water (pre-boiled) into a culture dish (31/2–41/2" diameter) and
add the organic material.
Some specific examples are:
Hay/Rice Medium—To the dish of pre-boiled water add two or three grains of rice and
three small (2") pieces of timothy hay. Let stand, uncovered, 24 to 48 hours and inoculate.
Recom mend ed for all types of amoeba, volvox and ciliates.
Wheat Medium—To the dish of pre-boiled water add four to six grains of pre-treated
wheat seed and let stand, uncovered, 24 to 48 hours. Inoculate with Blepharisma, all types
of Paramecia, Stentor or Vorticella.
For culturing non-specific organisms, prepared infusions can be inoculated with water (a
few mL) from any nearby pond, stream or puddle. See what develops!
ANIMALS continued on next page.
BIOLOGY ANIMALS
/?type=All&defaultType=Products&query=AB1423
/?type=All&defaultType=Products&query=AB1392
/?type=All&defaultType=Products&query=AB1423
/?type=All&defaultType=Products&query=AB1392
/?type=All&defaultType=Products&query=FB1438
/?type=All&defaultType=Products&query=FB1438
/?type=All&defaultType=Products&query=FB1820
/?type=All&defaultType=Products&query=M0171
/?type=All&defaultType=Products&query=M0171
/?type=All&defaultType=Products&query=FB1820
/flinnsci.com
/flinnsci.com